An expression cassette for targeting foreign proteins into chloroplasts
نویسندگان
چکیده
منابع مشابه
An optimized transit peptide for effective targeting of diverse foreign proteins into chloroplasts in rice
Various chloroplast transit peptides (CTP) have been used to successfully target some foreign proteins into chloroplasts, but for other proteins these same CTPs have reduced localization efficiencies or fail completely. The underlying cause of the failures remains an open question, and more effective CTPs are needed. In this study, we initially observed that two E.coli enzymes, EcTSR and EcGCL,...
متن کاملTransport of Proteins into Chloroplasts
The transport of proteins across the thylakoid membrane in higher plant chloroplasts is usually mediated by an amino-terminal peptide extension which is subsequently removed by a specific thylakoidal processing peptidase. We have previously shown that the reaction specificity of this enzyme is very similar to those of signal peptidases located in the endoplasmic reticulum and bacterial plasma m...
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We developed a method for introducing an N-glycosylation cassette into proteins at random sites by constructing cDNAs and expressing it in mammalian cells. The protocol entails four steps: (i) generation of cDNAs that contain single, randomly-located blunt end cuts; (ii) ligation of N-glycosylation cassettes into the blunt end cuts in three-frame formats; (iii) selection of the cDNA clones enco...
متن کاملEngineering chloroplasts: an alternative site for foreign genes, proteins, reactions and products.
Plant genetic engineering via the nucleus is a mature technology that has been used very productively for research and commercial biotechnology. By contrast, the ability to introduce foreign genes at specific locations on a chloroplast's chromosome has been acquired relatively recently. Certain limitations of nuclear genome transformation methods might be overcome by the site-specific introduct...
متن کاملTransport of proteins into mitochondria and chloroplasts
KEY WORDS post-translational transport precursor processing transit peptide organelle envelope. envelope receptors I. INTRODUCTION It has been well established that mitochondria and chloroplasts are not autonomous organeUes. These organelles are capable of nucleic acid and protein synthesis, but many soluble and membrane proteins that become localized in them are initially synthesized on cytopl...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1988
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/16.23.11380